Nelson Rodriquez

Food Safety and detection kits

The labs develop powerful alternatives for the evaluation of feed and food samples.
Food safety kits for diagnostic of kits

There’s a vast selection of diagnostic kits for food security & drug residues in food and feed. The labs develop powerful alternatives for the evaluation of feed and food samples. All these Elisa kits are specially designed to satisfy top quality requirements in food and feed testing focusing chiefly on chemical contaminants containing on artificial and natural contaminants. Our most important lines are antibiotics, mycotoxins and hormones analyzing solutions.

Over the previous twenty decades, exposure to toxic metals and mould is now hazardous and increasingly uncontrollable. This has caused the demand for greater consciousness of mycotoxin-producing fungi as potential pathogens in human disorder. It’s imperative to understand not just whether molds making mycotoxins have been in the middle of the men, but also if these molds and their toxins exist in his own body, which may possibly be the source of his health issues. Toxic metals like mercury, lead, aluminum have been Demonstrated to be a number of the main causes of health problems, like:

  • Depression
  • Diabetes
  • Neurological diseases – epilepsy and multiple sclerosis
  • Overweight
  • Weak immunity
  • High blood pressure

But how Can you know if your System is Vulnerable to toxic metals? This can be done quickly and easily with the Assistance of Mycotoxin Diagnostics and the Laboratory study of Toxic Metals and Minerals in the Detox Center.

What Mycotoxins Are, and how to find them?

Where the Mycotoxins could be found? Here is a short lost of some of the foods that may contain them.
Where the Mycotoxins could be found?

It’s estimated that there are more than 50,000 distinct species of mould, but only about 200 of these pose severe dangers to animal or human health. These dangerous species are known as toxic molds and therefore are potentially harmful as they can produce toxins called mycotoxins.

Mycotoxins are trichothecenes, aflatoxins, ochratoxins, gliotoxins, haetoglobosins and sterigmatocystin. When circumstances are suitable, the fungi multiply in colonies and mycotoxin levels become quite high. Toxins vary considerably in their seriousness: several mushrooms create severe toxins at particular levels of moisture, humidity or oxygen from the atmosphere; several toxins are mortal; some cause sickness or health issues; a few weaken the immune system without creating signs particular for this poison; some behave as allergens or irritants and a few have very little impact on people.


Mycotoxins are natural toxins produced by certain molds (fungi) and may be discovered in food. These kinds of life increase in a number of foods and crops, such as cereals, spices, nuts, dried fruits, apples and coffee beans, frequently in humid and warm conditions.

 Mycotoxins: types, effects and fungi  aflatoxins, ochratoxin A, patulin, fumonisins, zearalenone and nivalenol
Mycotoxins: types, effects and fungi

The most frequently observed mycotoxins that pose a threat to human health and livestock contain aflatoxins, ochratoxin A, patulin, fumonisins, zearalenone and nivalenol / deoxynivalenol. Mycotoxins show up in the food chain because of mould disease of these plants prior to and after the crop. Exposure to mycotoxins can occur either straight through the ingestion of contaminated foods, or from creatures that feed on contaminated feed, particularly milk.

What is? That the purpose of these Evaluations for toxic metals

The objective of these evaluations for mycotoxins and poisonous metals is to ascertain whether selected mycotoxins could be retrieved and found in human tissues and body fluids from individuals exposed to molds generating toxins from the environment.

Hazardous metal evaluations are performed with a urine sample. Their objective is to ascertain whether the individual’s body includes poisonous metals and mycotoxins and at what amounts.

Some of the most famous mycotoxins are sterigmatocystin, anatoxins and ochratoxin A.

Sterigmatocystin is a mycotoxin is known to be carcinogenic to the liver and digestive system. It may be found in household dust, and this can be most commonly deposited in furniture, as, and in some foods such as corn, cereals, cashews.

Aflatoxins draft under Electron Microscope
Aflatoxin

were assessed using immunosorbent columns comprising antibodies into the aflatoxin group (B1, B2, G1 and G2). Alfatoxins are seen in legumes, rice, corn, nuts. These toxins may be found in the milk of animals fed contaminated foods in the kind of aflatoxin M1. High levels of aflatoxins may result in severe poisoning (aflatoxicosis) and may be life threatening, usually by harm to the liver. Aflatoxins also have been shown to be genotoxic, meaning that they could damage DNA and lead to cancer in animal species. There’s also evidence that they can lead to liver cancer in people.

Ochratoxin A is Assessed using immunosorbent columns Comprising antibodies Ochratoxin A is shaped through the harvest of plants and is known to cause a range of toxic compounds in animal species. The sensitive and noticeable impact is kidney impairment, but the poison can have an impact on both the fetal development and the immune system in the event of pregnancy. In contrast to clear signs of renal toxicity and renal cancer because of ochratoxin A exposure in animals, this connection in humans is uncertain, but impacts on the liver have been shown.

Patulin is a mycotoxin derived from assorted molds, particularly Aspergillus, Penicillium and Byssochlamys. Regularly utilized in decayed apples and citrus goods, patulin may also look in a variety of molds and other foods. The key human nutrient elements of patulin are apples and citrus juice made from fruits. Acute creature symptoms include liver, spleen and kidney toxicity and damage to the immune system. Nausea, gastrointestinal ailments and nausea have been reported in humans.

Who are? these poisonous metals evaluations for?

Toxic metallic evaluations are acceptable for anybody who believes they could be vulnerable to mold or toxic metals in their everyday lives. Included in these are quite a high number of individuals:

  • Individuals working inside;
  • Individuals working with paints and varnishes every day;
  • Individuals working at a polluted industrial surroundings;
  • People who frequently eat certain kinds of fish (salmon, tuna);
  • Individuals who cook their meals in aluminum containers;
  • Consumption of fruits and vegetables treated with pesticides;

Individuals who regularly use varnishes, paints and other decorative items.

We’re subjected to a massive number of mycotoxins and poisonous metals daily. They could reach our own bodies not just in some specific professions but also with all the food that we eat and the air we breathe. Thus, tests for the detection of mycotoxins and toxic metals are acceptable for any man who would like to have a clearer notion of ​​the status of their own body. The outcomes of those tests can help you not only learn whether there are poisonous metals in our bodies, but also find out how to exclude them out of your everyday routine.

A mold that produces mycotoxins can develop on many different plants and foodstuffs and may penetrate deep into the meals, not just staying in the surface. Molds do not ordinarily grow in dried and stored meals, so powerful drying of their goods and keeping up their dry condition and appropriate storage is an effective step against mould growth and mycotoxin production.

To minimize the health risk of mycotoxins, humans are advised to:

  • Inspect whole grains (especially corn, sorghum, wheat, rice), dried figs and nuts such as peanuts, almonds, nuts, coconuts, Brazil nuts and As peanuts, nuts, almonds, coconuts, Brazil nuts and hazelnuts which are Frequently contaminated with aflatoxins;
  • Refrain from use of damaged cereals rather than save them, as Damaged grains are somewhat more inclined to mold strikes and consequently contamination with mycotoxins;
  • To select nuts and grains that will be rich and produced more recently;
  • Check the food is stored correctly – off from Insects, in arid and not overly warm areas;

The Way to Learn Your Toxic Metallic Test Success

Will find these outcomes:

  • Choosing the Toxic Metals and Mycotoxins test Accessible, You’ll find these outcomes:
  • Advice about 7 mycotoxins and 4 molds;
  • Detection of the smallest levels of mycotoxins in Your own body;
  • The harm they cause to the body

All of this with only 1 urine sample.

All of mycotoxin and thick metal evaluation results are formatted within an Easy-to-understand digital format revealing ppb (parts per billion) detection Amounts, or in other words, the focus level for the component within the body. The results also indicate if the evaluation is positive ambiguous or negative, Together with detection ranges for every.

HER-2 testing for breast cancer

What is HER2?

The human epidermal growth factor receptor 2 (HER2) gene accounts for producing HER2 proteins. HER2 proteins are found on the face of some breast cancer cells. When they’re triggered, they suggest breast cancer cells to multiply and divide. Usually, HER2 proteins modulate and manage the growth of breast cells. However, if the HER2 gene is mutated, that’s true in about 1 of 5 cases of breast cancer, it makes a lot of HER2 proteins. This leads to breast cells growing and dividing out of control.

HER2 Overexpresion Breast Cancer normal cell vs cancer cells
Breast Cancer Cell vs Normal Breast cell

Immunohistochemistry (IHC) tests and ISH for HER2 protein receptors testing?

Immunohistochemistry (IHC) tests are done to see if breast cancer cells have too many HER2 protein receptors. An in situ hybridization (ISH) test looks at the genetics of this sample, and also the results of the evaluation are also classified as positive, negative, or equivocal. An equivocal result requires further testing.

Sometimes IHC testing is completed . But if IHC testing is inconclusive, an ISH evaluation should be done. In many cases, an ISH test can confirm if the cancer is HER2-positive or HER2-negative.

Whether an initial ISH evaluation is inconclusive, an IHC may be performed or a repeat ISH evaluation on a new tissue sample could be required. Your doctor might want to get an extra biopsy to check another sample. Sometimes, both IHC and ISH tests may be needed to confirm HER2 status.

The immunohistochemistry (IHC) and in situ hybridization (ISH) services are supplied to customers with specific needs for the tissue array experiments. These analyses are conducted to localize the protein/gene of interest by using customer-supplied antibodies, DNA fragments or oligonucleotides on normal and diseased human tissues selected from tissue array lender. These services are highly cost-effective and ran to the highest standards to assist you in understanding the function of your genes/proteins or in identification and analysis of your potential diagnostic and therapeutic goals. The results are shown as a publishable, detailed pathology report followed by high-resolution digital photos.

Imunohisto Chemestry breast cancer
HER2+ and HER2-; different type of staining H&E ER PR
Different type of staining in IHC HER2 positive vs HER2 negative

What is FISH?

Fluorescence in situ hybridization (FISH) is an evaluation that”maps” the hereditary material in a individual’s cells. This evaluation may be used to visualize certain genes or parts of genes. The further copies of the HER2 receptor which are current, the greater HER2 receptors that the cells have. These HER2 receptors get signals that stimulate the development of breast cancer cells.

The FISH test results will inform you that the cancer is “favorable” or”adverse” (an outcome occasionally reported as”zero”) for HER2. FISH is considered more precise. Oftentimes, a lab is going to do the IHC test , ordering FISH only as long as the IHC results do not clearly show if the cells are either HER2-positive or negative.

IHC positive FISH positive
HER2 positive tested with FISH vs HER2 with ICH

 What is Whole Tissue Sections used for?

FFPE entire tissue sections are ideal candidates for localizing DNA, RNA and protein markers. The cells were fixed by formalin significantly less than 48 hours, then processed and sectioned. Two tissue segments using 5 µm (micro, micrometer) thickness is mounted onto a SuperFrost Plus glass slip. Paraffin tissue section is acceptable for detection of proteins and genes expression in certain tissues of various species.

Characteristics

• Range of tissue Species and types

• Suitable for the two immunohistochemistry (IHC) and in situ hybridization (ISH)

• 2 complete tissue Segments per slide

Applications

• Identification of Tumor markers and disease certain genes

• Detection of publication Enzymes and protein markers